制备不同分子量的几丁寡糖及其抗氧化活性

Ngo, D.-N., S.-H. Lee, et al. (2009). "Production of chitin oligosaccharides with different molecular weights and their antioxidant effect in RAW 264.7 cells." Journal of Functional Foods
译者：乔莹
韩国釜庆大学(Pukyong National University)的海洋生物过程研究中心(Marine Bioprocess Research Center)的最新研究发表在Journal of Functional Foods上。该实验室在Se-Kwon Kim的领导下，一直致力于壳寡糖及其相关产品的应用研究，共发表英文论文30余篇。
该实验室采用酸水解加膜超滤技术，将脱乙酰度<10%的几丁质降解为分子量1-3kDa及小于1kDa的几丁寡糖，并进行质谱鉴定。MTT分析表明，2种几丁寡糖在1mg/ml浓度下对巨噬细胞株RAW264.7均无细胞毒作用。细胞暴露在羟自由基中会导致氧化性损伤，2种几丁寡糖均能抑制这种氧化损伤, 清除胞内过量ROS的产生，提高胞内谷胱甘肽的水平。并且分子量1-3kDa的几丁寡糖对自由基的清除效果要优于分子量小于1kDa的。这些结果表明，2种几丁寡糖均具有较好的抗氧化活性，可用于日常生活中对抗氧化压力。

Abstract：
The purpose of this research is not only to produce two kinds of chitin oligosaccharides or N-acetyl chito-oligosaccharides (NA-COSs) with different molecular weights (MW) from crab chitin hydrolysis solution but also to determine their effect against oxidative stress in live cells. Two kinds of NA-COSs with MW 1–3 kDa (NA-COS 1–3 kDa) and below 1 kDa (NA-COS < 1 kDa) were obtained using an ultrafiltration membrane system. They exhibited an inhibitory effect against DNA and protein oxidation. In addition, in their presence, intracellular glutathione (GSH) level and direct intracellular radical scavenging effect were significantly increased in a time-dependent manner in mouse macrophages (RAW 264.7) and rendered inhibitory effect against cellular oxidative stress. In particular, NA-COS 1–3 kDa was more effective than NA-COS < 1 kDa in protein oxidation and production of intracellular free radicals in live cells. These results suggest that NA-COSs act as a potential scavenger against oxidative stress in cells.